Fig. 3

Knockdown of miR-33a-5p enhances the functions of pancreatic β cells. a MiR-33a-5p inhibitor and inhibitor NC were transfected into INS-1 cells for 48 h, followed by qRT-PCR. n = 3. b INS-1 cells were transfected with miR-33a-5p inhibitor or inhibitor NC for indicated time, followed by CCK-8 assay. n = 6 for each time point. MiR-33a-5p inhibitor and inhibitor NC were transfected into INS-1 cells for 48 h. Insulin content (c) and insulin secretion (d) were determined by ELISA assay. n = 6. ^*p < 0.05, ^**p < 0.01