Fig. 2
From: Inhibition of germinal vesicle breakdown using IBMX increases microRNA-21 in the porcine oocyte
Inhibition of GVBD Increased MIR21 Abundance. Collected COCs underwent IVM in the presence of a vehicle control or 1.0 mM IBMX, and then collected and denuded at 8 h intervals. The oocytes that underwent IVM in the presence of IBMX had approximately 30-fold greater MIR21 abundance at 24 h of IVM compared to control oocytes (a). This increase of MIR21 at 21 h of IVM was temporally associated with decreased PDCD4 abundance (b) suggesting the increased MIR21 due to IBMX prevented GVBD is biologically active within the oocyte. Asterisks denote significant difference (P < 0.05)