Fig. 6

The effect of PGE₂ (10 μM) on oxidative stress in bovine oocytes after IVM. Panel (A) depicts representative fluorescent images of intracellular GSH (a, c) and ROS (b, d) levels in the control (a, b) and PGE₂-treated (c, d) oocytes. Bars = 50 μm. Panels (B) and (C) depict quantitative analysis of PGE₂ effect on intracellular GSH and ROS levels, respectively. The values are presented as an average fluorescence intensity and expressed as mean ± SEM of three independent repeats (25 COCs per treatment group). Different letters indicate significant differences (P < 0.05), as determined by Student’s t-test. Panels (D) and (E) depict relative mRNA levels of the antioxidation-associated genes: CAT and GPX4, respectively, in the control and PGE₂-treated oocytes. The values are presented as arbitrary units and expressed as mean ± SEM of eight independent repeats (10 oocytes in each replicate). Different letters indicate significant differences (P < 0.05), as determined by Student’s t-test