Table 1 The primer sequences of the RT-qPCR tested genes
Gene | Primer forward (5′ to 3′) | Primer reverse (5′ to 3′) |
|---|---|---|
Bmal1 (rat) | GGCTGTTCAGCACATGAAAAC | GCTGCCCTGAGAATTAGGTGTT |
Clock (rat) | CTTCCTGGTAACGCGAGAAAG | GTCGAATCTCACTAGCATCTGAC |
Per1 (rat) | GATGTGGGTGTCTTCTATGGC | AGGACCTCCTCTGATTCGGC |
Per2 (rat) | CAGGTTGAGGGCATTACCTCC | AGGCGTCCTTCTTACAGTGAA |
Cry1 (rat) | CACTGGTTCCGAAAGGGACTC | CTGAAGCAAAAATCGCCACCT |
Cry2 (rat) | CACTGGTTCCGCAAAGGACTA | CCACGGGTCGAGGATGTAGA |
Sirt1 (rat) | TGATTGGCACCGATCCTCG | CCACAGCGTCATATCATCCAG |
Nampt (rat) | CCTGGTATCCAATTACAGTGGC | CCAAATGAGCAGATGCCCCTAT |
Glut4 (rat) | ACACTGGTCCTAGCTGTATTCT | CCAGCCACGTTGCATTGTA |
Pparg (rat) | GGAAGACCACTCGCATTCCTT | GTAATCAGCAACCATTGGGTCA |
β-Actin (rat) | GGCCAACCGTGAAAAGATGACC | AACCCTCATAGATGGGCACAG |
Bmal1 (mouse) | ACAGTCAGATTGAAAAGAGGCG | GCCATCCTTAGCACGGTGAG |
Clock (mouse) | ATGGTGTTTACCGTAAGCTGTAG | CTCGCGTTACCAGGAAGCAT |
Per1 (mouse) | GAATTGGAGCATATCACATCCGA | CCCGAAACACATCCCGTTTG |
Per2 (mouse) | CTCCAGCGGAAACGAGAACTG | TTGGCAGACTGCTCACTACTG |
Sirt1 (mouse) | ATGACGCTGTGGCAGATTGTT | CCGCAAGGCGAGCATAGAT |
Nampt (mouse) | GCAGAAGCCGAGTTCAACATC | TTTTCACGGCATTCAAAGTAGGA |
Glut4 (mouse) | GGACCGGATTCCATCCCAC | TCCCAACCATTGAGAAATGATGC |
Pparg (mouse) | CTCCAAGAATACCAAAGTGCGA | GCCTGATGCTTTATCCCCACA |
β-Actin (mouse) | GTGACGTTGACATCCGTAAAGA | GCCGGACTCATCGTACTCC |