Fig. 1

Glucose, Insulin T and Insulin 2 levels in serum, interstitial tissue- (ITf) and seminiferous tubule-enriched (STf) fractions (a-e) and insulin receptor subunits β and α levels in ITf and STf (h and i) during mouse postnatal development. For measurements in serum samples, three animals per age group were used. For measurements in tissue samples, five animals were used per age group. Values shown are the mean ± SEM. a and b glucose concentration expressed a in mg/dl in serum and b in μg/mg respectively in ITf and STf from 14 to > 60 days (d) postpartum in normal mice. a Circulating glucose concentrations significantly increased in (***P < 0.0005) 21d (d) versus (vs) 14d then, (‡P < 0.03) in 28 vs 21d and again (##P < 0.002) in 42 vs 28d. b The glucose concentration was significantly augmented in ITf (#P < 0.02) in 21 vs 14d (***P < 0.0005) then, in 28 vs 21d whereas in STf, the rise was significant (#P < 0.02) in 42 vs 14d and (‡P < 0.03) in 42 vs 28d. c In the serum, insulin T significantly decreased (**P < 0.005) in the 21, (***P < 0.0005) 28- and (†P < 0.01) and 42-d-old mice whereas insulin 2 significantly diminished in the (*P < 0.05) 21- and (**P < 0.005) and 28-d-old mice. d The rise in Insulin T is significant (##P < 0.002) from 42 to > 60d while the decrease in Insulin 2 is gradual from 14 to > 60 days. e Insulin T and Insulin 2 values and profiles are similar, both exhibited a sharp and very significant increment (aP < 0.00001) from 28 to 42 days and again (††P < 0.001) in the > 60-d-old mice. f and g representative western blots accompanied by corresponding histograms of the IR-β subunit in f ITf and h STf from 14 to > 60 d. f the changes in 110 kDa are significant by (††P < 0.001) 21, 28, 35, 42 and (##P < 0.002) > 60 days; the changes in 98 kDa are significant by (# < P < 0.02) 21, (†P < 0.01) 28 and (##P < 0.002) 35 days; the changes in 75 kDa are significant by (##P < 0.002) 21, (††P < 0.001) 28, 35, 42 and (aP < 0.0001) > 60 days; the changes in Total IR-β levels are significant in (††P < 0.001) 21, 28, 35, 42, and > 60 day-old mice. g the changes in 110 kDa are significant by (††P < 0.001) 21, 28, 35 and 42 days; the changes in 98 kDa are significant by (††P < 0.001) 21, 35, 42 and by (†P < 0.01) 28 and > 60 days. h and i representative western blots with corresponding histograms of IR-α subunit in h ITf and i STf from 14 to > 60 days after birth. h the changes in (bP < 0.00005) 135-, 90-, (†P < 0.03) 75-, (**P < 0.005) 50-, (###P < 0.0002) 36-, (kP < 0.00002) 31 kDa and (bP < 0.00005) Total IR-α levels are significant in the 28-day-old mice; the changes in (###P < 0.0002) 135-, 90-, (#P < 0.05) 75-, 31-, (bP < 0.00005) 36 kDa and (***P < 0.0005) Total IR-α levels are significant in the 42-day-old mice; the changes in (‡P < 0.03) 135-, (‡‡‡P < 0.0003) 90-, (##P < 0.002) 75-, (##P < 0.0002) 50-, (**P < 0.005) 36-, (‡P < 0.03) 31 kDa and (##P < 0.002) Total IR-α levels in the > 60-day-old mice are significant. i the changes in (††P < 0.001) 135-, (#P < P < 0.02) 90-and (#P < 0.02) 36 kDa are significant in the 21-day-old mice; the changes in (*P < 0.02) 75-, (‡P < 0.03) 50-, (##P < 0.002) 36 kDa and (**P < 0.005) Total IR-α levels are significant in the 28-day-old mice; the changes in (†P < 0.01) 75- and (***P < 0.0005) 36 kDa are significant in the 42-day-old mice; the changes in (*P < 0.05) 135-, (###P < 0.0002) 90-, (#P < 0.02) 75-, (‡P < 0.03) 36-, (†P < 0.01) 31 kDa and Total IR-α levels in the > 60-day-old mice