Table 2 Details of primary antibodies used for western blot and immunohistochemistry
From: Disruption of androgen signaling during puberty affects Notch pathway in rat seminiferous epithelium
Antibody | Host species | Vendor | Cat. Number | Dilution |
|---|---|---|---|---|
Anti-actin | Mouse | Sigma-Aldrich | A2228 | 1:3000 (WB)a |
Anti-DLL1 | Rabbit | Sigma-Aldrich | SAB2100593 | 1:1000 (WB), 1:100 (IHC) |
Anti-DLL4 | Rabbit | Abcam | AB7280 | 1:2000 (WB), 1:400 (IHC) |
Anti-HES1 | Rabbit | Thermo Fischer Scientific | PA5–28802 | 1:1000 (WB); 1:100 (IHC) |
Anti-HES5 | Goat | Santa Cruz Biotechnology | sc-13,859 | 1:300 (WB), 1:50 (IHC) |
Anti-HEY1 | Rabbit | Thermo Fischer Scientific | PA5–40553 | 1:500 (WB), 1:100 (IHC) |
Anti-JAG1 | Rabbit | Thermo Fischer Scientific | PA5–72843 | 1:3000(WB), 1:600 (IHC) |
Anti-LHR | Rabbit | Santa Cruz Biotechnology | sc-25,828 | 1:1000 (WB), 1:200 (IHC) |
Anti-N1ICD | Rabbit | Abcam | Ab8925 | 1:1000 (WB), 1:200 (IHC) |
Anti-N2ICD | Rabbit | Sigma-Aldrich | SAB4502020 | 1:500 (WB), 1:200 (IHC) |