Fig. 5

Effect of flutamide and EDS on N1ICD and N2ICD immunoexpression in peripubertal rat testis. Representative micrographs are obtained from three independent analyses. Images A – C and E – G represent early stages of seminiferous epithelium cycle, images A’ – C′ and E’ – G’ represent late stages of seminiferous epithelium cycle, images A” – C″ represent interstitial tissue. Negative control included section incubated with non-immune serum instead of the primary antibody (insert in C). Scale bar = 20 μm. Positive signals of the proteins are depicted by arrows. Sertoli cells – arrows, spermatogonia – arrowheads, preleptotene spermatocytes – open arrows, leptotene/zygotene spermatocytes - double arrows, early pachytene spermatocytes - short white arrows, late pachytene spermatocytes – short arrows, round spermatids – white arrows, elongated spermatids – white arrowheads, Leydig cells – asterisks. Quantitative analysis of the intensity of immunocytochemical stainings expressed as relative optical density (ROD) of diaminobenzidine brown reaction product (D, H). The histograms are the quantitative representation after densitometry of data (mean ± SD) of three independent analyses. Significant differences from control values are denoted as *p < 0.05, and **p < 0.01. Spg – spermatogonia, PL/L/Z Spt - preleptotene/leptotene/zygotene spermatocytes, Early PSpt - early pachytene spermatocytes, Late PSpt - late pachytene spermatocytes, Rspd – round spermatids, Espd – elongated spermatids