Fig. 4

Effect of flutamide (F) and EDS on Notch1 and Notch2 expression in peripubertal rat testis. (A, B) Relative expression of Notch1 and Notch2 mRNAs was determined using real-time RT-PCR analysis. The histograms are the quantitative representation of data of three independent analyses (n = 6 each group). The expression values of the individual genes were normalized to the mean expression of the reference genes (Rn18s, B2m and Actb) as an internal control. Relative quantification (RQ) is expressed as mean ± SD. Significant differences from control values are denoted as **p < 0.01 and ***p < 0.001. (A’, B′) Relative protein levels of N1ICD and N2ICD were determined using western blot. The histograms are the quantitative representation after densitometry of data (mean ± SD) of three independent analyses (n = 6 each group). The relative level of studied protein was normalized against its corresponding actin data point. The protein levels within the control group were arbitrarily set as 1. Significant differences from control values are denoted as *p < 0.05, **p < 0.01, and ***p < 0.001