Fluorescent analysis of boar sperm capacitation process in vitro

Table 2 Correlation matrix of individual detection methods of boar sperm (chilled 17 °C/diluted) capacitation status at 240 min of incubation; n = 20

	ACR.2 FM	ACR.2 FC	CTC FM	CTC FC	Phall FM	Phall FC	pY FM	pY FC
ACR.2 FM^a	1	0.79	0.81	0.19	0.33	0.55	0.47	0.55
ACR.2 FC^b	0.79	1	0.69	0.17	0.33	0.62	0.39	0.44
CTC FM^c	0.81	0.69	1	0.25	0.35	0.59	0.52	0.47
CTC FC^d	0.19	0.17	0.25	1	0.24	0.31	0.12	0.23
Phall FM^e	0.33	0.33	0.35	0.24	1	0.47	0.27	0.35
Phall FC^f	0.55	0.62	0.59	0.31	0.47	1	0.23	0.29
anti-pY FM^g	0.47	0.39	0.52	0.12	0.27	0.23	1	0.71
anti-pY FC^h	0.55	0.44	0.47	0.23	0.35	0.29	0.71	1
r totalⁱ	4.69	4.43	4.68	2.51	3.34	4.06	3.71	4.04

Correlation coefficients (r) between detections of capacitation status by individual detection methods
^aFluorescent microscopy with anti-acrosin (ACR.2 FM) antibody
^b Flow cytometry with ACR.2 antibody (ACR.2 FC)
^cFluorescent microscopy with chlortetracycline (CTC FM)
^dFlow cytometry with chlortetracycline (CTC FC)
^eFluorescent microscopy with fluorescein isothiocyanate-conjugated phalloidin (Phall FM)
^fFlow cytometry with fluorescein isothiocyanate-conjugated phalloidin (Phall FC)
^gFluorescent microscopy with anti-phosphotyrosine antibody (anti-pY FM)
^hFlow cytometry with anti-phosphotyrosine antibody (anti-pY FC)
ⁱThe sum of correlation coefficients for appropriate detection method
Significant correlation coefficient in bold (p ≤ 0.05)

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