Table 1 Specific fluorescent patterns of the boar sperm (chilled 17 °C/diluted) as detected by individual fluorescent methods
From: Fluorescent analysis of boar sperm capacitation process in vitro
Assay | chlortetracycline (CTC) | anti-acrosin antibody (ACR.2) | anti-phosphotyrosine (pY) | fluorescein isothiocyanate-conjugated phalloidin (FITC-phall) |
|---|---|---|---|---|
Non-capacitated, acrosome intact sperm | Bright fluorescence over the entire sperm head and positive mid-piece of the tail | Moderate fluorescence in the acrosome | Low fluorescence in the sperm head, triangular segment within the head and tail | Moderate fluorescence in the sperm head and tail |
Capacitated, acrosome-intact sperm | Prominent fluorescence in the equatorial segment, mid-piece of the tail and no fluorescence (dark) in the post-acrosomal region of the head | Intensive fluorescence in the acrosome | Intensive fluorescence in the sperm head, triangular segment within the head and tail | Intensive fluorescence in the acrosome and tail |
Acrosome-reacted sperm | Low fluorescence over the sperm head, with remaining positive signal in the equatorial segment and mid-piece of the tail | Low or no fluorescence in the sperm head with a remaining fluorescence in the equatorial segment of the sperm head | No fluorescence in the acrosome with a remaining positive fluorescence in the triangular segment within the head and tail | Low fluorescence in the sperm head with a remaining positive signal in the tail |