Fig. 6

Time-dependent short-term effect of EGF on the expression of Hippo proteins in COCs a. Immunoblot showing pYAP (Ser127) and β-actin in COCs treated with EGF (10 ng/ml) for 0 and 60 min. b. Densitometric ratio of YAP1 relative to β-actin, N = 3–4. c. Immunoblotting showing pWWTR1 (Ser89) and β-actin in COCs treated with EGF (10 ng/ml) for 0, 10, 30 min and 1.5 h. d. Densitometric ratio of pWWTR1 relative to β-actin. N = 3–4, 30 min time point was excluded from analysis because there were only 2 observations. Values are mean ± SEM. ^* indicate significant differences by student’s t-test, P < 0.05