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Table 1 Primary Antibodies and Dilutions Used

From: Molecular analysis of the effects of steroid hormones on mouse meiotic prophase I progression

Primary Antibody

Company

Catalog #

Species Made In

Dilution

MSY2 (C-15)

Santa Cruz Biotechnology

sc-21,316

Lot #: C0615

Goat

1:100

SYCP3 (D-1)

Santa Cruz Biotechnology

sc-74,569

Lot #: J1314

Mouse

1:200 (whole mount) 1:50 (surface spreads)

TRA98

B-Bridge

73–003

Lot #: 141113A

Rat

1:500

VASA

Abcam

ab-13,840

Lot #: GR172688–1

Rabbit

1:250