Fig. 3

MiR-322 downregulation promoted GC-2 cell apoptosis. a Cell apoptosis was analyzed by flow cytometry. Early apoptosis and total apoptosis rates in the experimental group were markedly higher (5.12% vs 13.92%; 6.5% vs 17.5%) than those of the control group (P < 0.01) (b) Relative expressions of caspases 3, 9, and 8, Bax, and Bcl-2 at the mRNA level were determined by quantitative RT-PCR using β-actin as the internal control. c, d Relative expressions of caspases 3, 9, and 8, Cleaved caspases3 and 9,Bax, and Bcl-2 at the protein level were determined by Western blot using GAPDH as the internal control. Compared with the control group, the mRNA expression levels of caspases 3, 9, 8 and Bax in the experimental group were 1.27% vs 3.37,1.11% vs 6.37,0.95% vs 2.22 and 1.04% vs 2.15%, respectively. by contrast, Bcl-2 expression significantly decreased (1.02% vs 0.33%,P < 0.05) compared with that in the control group.Each bar presents the mean ± SEM of three independent experiments for each group (^*P < 0.05, ^** P < 0.01)