Cell-free mitochondrial DNA in human follicular fluid: a promising bio-marker of blastocyst developmental potential in women undergoing assisted reproductive technology

Table 2 Relationship of the cf-nDNA and the relative cf-mtDNA (cf-ND1/cf-β-globin ratio) levels in human follicular fluid with embryo development outcomes

FF associated with	n of FF(%)	cf-nDNA (ng/ml)			relative cf-mtDNA
FF associated with	n of FF(%)	MD (IQR)	OR (95% CI)	p	MD (IQR)	OR (95% CI)	p
High-quality embryos	89 (62.68)	0.23 (0.13–0.50)	0.94 (0.78, 1.12)	0.46	5.27 (2.99–9.67)	1.00 (0.95, 1.05)	0.94
poor-quality embryos	53 (37.32)	0.24 (0.13–0.32)	0.94 (0.78, 1.12)	0.46	7.39 (4.14–10.07)	1.00 (0.95, 1.05)	0.94
blastocysts	22 (51.16)	0.63 (0.21–1.81)	26.43 (0.75, 929.72)	0.071	2.84 (1.53–6.99)	0.87 (0.76, 0.99)	0.030
No blastocysts	21 (48.84)	0.14 (0.08–0.25)	26.43 (0.75, 929.72)	0.071	7.80 (4.66–10.34)	0.87 (0.76, 0.99)	0.030

Percentages are calculated within groups. Data are presented as MD (IQR) and are analyzed using multivariate logistic regression. Statistical significance was assumed at P < 0.05. FF follicular fluid, MI metaphase I, MII metaphase II, GV germinal vesicle, OR odds ratio, CI confidence interval, MD Median, IQR interquartile range

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