Entry into puberty is reflected in changes in hormone production but not in testicular receptor expression in Atlantic salmon (Salmo salar)

Table 1 Forward and reverse primers, and TaqMan fluorogenic (FAM-TAMRA or FAM-MGBNFQ) probes, used in real-time, quantitative PCR assays to determine the relative ara1 and ara2 mRNA levels in Atlantic salmon.

Target	GenBank accession no.	Primer^a	Sequence (5′➔3′)^a	Slope^b
ara1	KJ584693	ara1-Fw	CGCAGAATTGCCCTTAAGCA	−3,54
		ara1-Rv	CCACCATGAAGGCGAAAATG
		ara1-probe	FAM-CACAGACAACAGGGAAAAATAGCCACGATT-TAMRA
ara2	KJ584694	ara2-Fw	TGTCGCTCTGGACCGTTAGC	−3,60
		ara2-Rv	ATGGTGGTGCAGGCAGAATT
		ara2-probe	FAM-CAGTGCTGTCAACCAGCTGTGCCC-TAMRA

^aSequences are shown for the sense (Fw) and antisense (Rv) primers, and the TaqMan probe (probe). Probes have a 6-FAM label at their 5′-ends, and a TAMRA quencher label at their 3′-ends
^bSlope for primer/probe combinations obtained in validation experiments using four 10-fold dilutions of testis cDNA

ISSN: 1477-7827