Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12958-019-0480-0/MediaObjects/12958_2019_480_Fig5_HTML.png)
Analysis of mesenchymal stem cell markers by flow cytometry. (A) Flow cytometry histograms. (a) and (b): subcutaneous fat; (c) and (d): visceral fat from sham and EMS mice. Mesenchymal stem cell markers: Sca-1+, CD140b+, CD146+ . (B) Quantification of mesenchymal stem cell markers by flow cytometry. The number of Sca-1+ and CD146+ expressing stem cells are significantly lower in sham-visc, EMS-s.c and EMS-visc groups compared to sham-visc. EMS-visc has low Sca-1+ population compared to EMS-s.c group. Each bar represents the mean ± SE for data from three individual experiments and each experiment was performed in duplicate. * denotes statistical significance (p < 0.05) between sham-s.c vs other groups, EMS-s.c vs EMS-visc (Sca-1+)