Table 1 The primer sequences used for Real-time RT-PCR
From: miR-34c disrupts spermatogonial stem cell homeostasis in cryptorchid testes by targeting Nanos2
Gene | Species | Forward | Reverse |
|---|---|---|---|
Nanos2 | Mouse | CCATATGCAACTTCTGCAA | ACTGCTGACTGCTGTTGAGTG |
Nanos2 | Human | CTGAGAAGTGCCTACTCAAG | TGATACGGTGCTCTCCAGAG |
miR-34c | Human/Mouse | CACGCAAGGCAGTGTAGT | CCAGTGCAGGGTCCGAGGTA |
18S | Human/Mouse | CGGCGACGACCCATTCGAAC | GAATCGAACCCTGATTCCCCGTC |