Fig. 4

Effects of 4-PBA and 3-MA on ovarian histology in CDDP-treated mice. Mice were treated with saline, CDDP, and CDDP with 4-PBA or 3-MA for 1 day, 3 days and 7 days, respectively. a H&E staining showed the ovarian histology in each group. Five sections (taken 100 μm apart) from an ovary were photographed for follicle assessment. Scale bars = 40 μm (upper panels) and 4 μm (lower panels). b Comparison of the total number of healthy and atresia follicles among groups. Healthy follicles include healthy primordial, primary, secondary and antral follicles. c Comparison of the number of healthy primordial, primary, secondary and antral follicles among groups. n = 5, * P < 0.05, ** P < 0.01