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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Pharmacological sympathetic denervation prevents the development of polycystic ovarian syndrome in rats injected with estradiol valerate

Fig. 3

Immunofluorescence staining of tyrosine hydroxylase (TH) in ovaries of rats injected with a Vh or b EV at 14 days of age, c denervated with GTD (from days 7 to 27) + Vh at day 14 of life or d GTD (from days 7 to 27) + EV at day 14 of life, e Vh-injected at day 14 of life + GTD (from days 14 to 34) or f EV-injected at day 14 of life + GTD (from days 14 to 34), g Vh-injected at day 14 of life + GTD (from days 70 to 90) or h EV-injected at day 14 of life + GTD (from days 70 to 90). Ovarian sections were stained with anti-TH antibody (green color), and nuclear staining with DAPI (blue color). F: follicles in different stages of maturation; PC: pre-cyst; gr: granulosa cells; t: thecal cells

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