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Table 4 Characteristics of datasets that addressed the relationship between the induced AR% and FR

From: Human sperm acrosome function assays are predictive of fertilization rate in vitro: a retrospective cohort study and meta-analysis

First author and year Country Design N Preparation method Inducer Assay method FR cut-off value AR cut-off value Outcome
Fénichel 1991
[47]
France Prospective cohort 41 Swim up A23187 GB24 >  0% vs. = 0%
/= 0%
Spontaneous AR% ≥ 11.6% and/or Induced AR% < 20.6% /
Pampiglione 1993
[23]
UK Prospective cohort 54 Swim up A23187 Triple stain >  0% vs. = 0%
/= 0%
<  31.3% /
Parinaud 1993a
[37]
France Prospective cohort 53 Discontinuous gradient A23187 FITC-PNA >  0% vs. = 0%  
Parinaud 1993b
[37]
France Prospective cohort 53 Discontinuous gradient A23187 GB24 >  0% vs. = 0%  
Henkel 1993
[59]
Germany Prospective cohort 74 Swim up Low temperature Triple stain <  50% ≤ 10%
Calvo 1994
[25]
USA Prospective cohort 82
/232
No preparation HFF FITC-PSA Wholea/= 0% ≤ 5% /
Yovich 1994
[26]
Australia Prospective cohort 52 Swim up
/Discontinuous gradient
A23187 FITC-PSA = 0% <  10%
Parinaud 1995a
[41]
Monaco Prospective cohort 117 Discontinuous gradient A23187 FITC-GB24 Whole
/>  0% vs. = 0%
  /
Parinaud 1995b
[41]
Monaco Prospective cohort 117 Discontinuous gradient P FITC-GB24 Whole
/>  0% vs. = 0%
  /
Parinaud 1995c
[41]
Monaco Prospective cohort 117 Discontinuous gradient HFF FITC-GB24 Whole
/>  0% vs. = 0%
  /
Parinaud 1995d
[41]
Monaco Prospective cohort 117 Discontinuous gradient CAMP FITC-GB24 Whole
/>  0% vs. = 0%
  /
Parinaud 1995e
[41]
Monaco Prospective cohort 117 Discontinuous gradient TPA FITC-GB24 Whole
/>  0% vs. = 0%
  /
Sukcharoen 1995
[38]
UK Prospective cohort 41 Discontinuous gradient A23187 FITC-PNA >  0% vs. <  50%  
Parinaud 1995a
[42]
Monaco Prospective cohort 131 Discontinuous gradient A23187 FITC-GB24 >  0% vs. = 0%  
Parinaud 1995b
[42]
Monaco Prospective cohort 131 Discontinuous gradient TPA FITC-GB24 >  0% vs. = 0%  
Brandelli 1995
[27]
Argentina Prospective cohort 31 Discontinuous gradient BSA-GlcNAc/A23187 FITC-PSA Whole
/≥ 30% vs. <  30%
/<  30%
<  0.2b //
Krausz 1996a
[39]
Italy Prospective cohort 53/51
/52
Swim up A23187 FITC-PNA Whole
/≥ 50% vs. <  50%
/= 0%
≤ 10% //
Krausz 1996b
[39]
Italy Prospective cohort 59
/59
/60
Swim up P FITC-PNA Whole
/≥ 50% vs. <  50%
/= 0%
≤ 7% //
Carver-Ward 1996
[48]
Netherlands Prospective cohort 129 Discontinuous gradient A23187 Anti-CD46
antibody
Whole
/≤ 30%
≤ 10% /
Benoff 1997
[43]
USA Prospective cohort 58 Swim up Mannose RITC-PSA Whole
/≥ 63% vs. <  63%
/<  63%
≤ 0.1c //
Liu 1998
[28]
Australia Prospective cohort 109 Discontinuous gradient A23187 FITC-PSA Whole
/>  50% vs. ≤ 50%
  /
Rufas 1998
[64]
Israel Prospective case-control 62 Discontinuous gradient HFF FITC-PSA >  0% vs. = 0%  
Fukui 2000
[30]
Japan Prospective cohort 39 Discontinuous gradient P FITC-PSA >  0% vs. = 0%  
Esterhuizen 2001
[31]
South Africa Prospective cohort 35 Swim up ZP FITC-PSA Whole
/≤ 60%
≤ 15% /
Bastiaan 2003
[63]
South Africa Prospective cohort 30 Swim up ZP FITC-PSA >  50% vs. <  50%
/≤ 50%
<  8% /
Liu 2003
[32]
Australia Prospective cohort 65 Swim up ZP FITC-PSA Whole
/<  30%
≤ 16% /
El-Ghobashy 2003 [33] UK Prospective cohort 75 Swim up HFF FITC-PSA Whole
/≥ 50% vs. <  50%
  /
Katsuki 2005
[34]
Japan Prospective cohort 133 Swim up A23187 FITC-PSA >  0% vs. = 0%
/= 0%
<  21% /
Jędrzejczak 2005
[24]
Poland Prospective cohort 79 Discontinuous gradient A23187 Triple stain Whole  
Abu 2012
[35]
South Africa Prospective cohort 78 Double Swim up ZP FITC-PSA Whole  
  1. AR acrosome reaction, FR fertilization rate, HFF human follicle fluid, P progesterone, ZP zona pellucida, CAMP cyclic adenosine 3′-5′-phosphate analogue, TPA phorbol ester, BSA-GlcNAc Neoglycoproteins with N-acetylglucosamine residues, FITC-PNA fluorescein isothiocyanate-conjugated peanut agglutinin, FITC-PSA fluorescein isothiocyanate-conjugated Pisurn sativum agglutimm, FITC-GB24 fluorescein isothiocyanate-GB24, RITC-PSA rhodamine-conjugated Pisurn sativum agglutimm
  2. aAll infertile patients undergoing in vitro fertilization (IVF) therapy
  3. bBSA-GlcNAc induced increased %AR/A23187 induced increased %AR
  4. cMannose induced increased %AR/Mannose induced total %AR
  5. spearman correlation coefficient; induced AR% for higher and lower FR; binary diagnostic accuracy data as 2 × 2 tables