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Table 3 Characteristics of datasets that addressed the relationship between the spontaneous AR% and FR

From: Human sperm acrosome function assays are predictive of fertilization rate in vitro: a retrospective cohort study and meta-analysis

First author and year

Country

Design

N

Preparation method

Assay method

FR cut-off value

AR cut-off value

Outcome

Fénichel 1991 [47]

France

Prospective cohort

41

Swim up

GB24

>  0% vs. = 0%

 

â‘¡

Takahashi 1993 [40]

Japan

Prospective cohort

45

Discontinuous gradient

FITC-Con A

<  50%③

%AR ≥ 10% at 2 h or ≤ 5% at 4 hâ‘¢

â‘¢

Parinaud 1993a [37]

France

Prospective cohort

53

Discontinuous gradient

FITC-PNA

>  0% vs. = 0%②

 

â‘¡

Parinaud 1993b [37]

France

Prospective cohort

53

Discontinuous gradient

GB24

>  0% vs. = 0%②

 

â‘¡

Parinaud 1995 [41]

Monaco

Prospective cohort

117

Discontinuous gradient

F1TC-GB24

Wholea

 

â‘ 

Parinaud 1995 [42]

Monaco

Prospective cohort

131

Discontinuous gradient

FITC-GB24

>  0% vs. = 0%②

 

â‘¡

Krausz 1996

[39]

Italy

Prospective cohort

59

Swim up

FITC-PNA

≥ 50% vs. <  50%②

 

â‘¡

Hershlag 1997

[44]

USA

Prospective cohort

74

Swim up

TRITC-PSA

Wholeâ‘ 

 

â‘ 

Fujino 1997

[22]

Japan

Prospective cohort

30

Discontinuous gradient + Swim up

Two stain (new-methylene blue and cresyl violet) Blutstan kit

Wholeâ‘ 

 

â‘ 

Rufas 1998

[64]

Israel

Prospective cohort

62

Discontinuous gradient

FITC-PSA

>  0% vs. = 0%②

 

â‘¡

Kawamoto 1999

[29]

Japan

Prospective cohort

34

Swim up

MH61

>  0% vs. = 0%②

 

â‘¡

Bastiaan 2003

[63]

South Africa

Prospective cohort

30

Swim up

FITC-PSA

>  50% vs. <  50%②

 

â‘¡

El-Ghobashy 2003

[33]

UK

Prospective cohort

75

Swim up

FITC-PSA

≥ 50% vs. <  50%②

 

â‘¡

Wiser 2014

[36]

Israel

Prospective cohort

40

Not reported

FITC-PSA

≤ 35%③

≤10%③

â‘¢

  1. AR acrosome reaction, FR fertilization rate, FITC-ConA fluorescein isothiocyanate-conjugated Concanavalin A lectin, FITC-PNA fluorescein isothiocyanate-conjugated peanut agglutinin, FITC-GB24 fluorescein isothiocyanate-GB24, TRITC-PSA tetramethylrhodamine-conjugated Pisurn sativum agglutimm, FITC-PSA fluorescein isothiocyanate-conjugated Pisurn sativum agglutimm
  2. aAll infertile patients undergoing in vitro fertilization (IVF) therapy
  3. ① spearman correlation coefficient; ② spontaneous AR% for higher and lower FR; ③ binary diagnostic accuracy data as 2 × 2 tables