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Table 3 Characteristics of datasets that addressed the relationship between the spontaneous AR% and FR

From: Human sperm acrosome function assays are predictive of fertilization rate in vitro: a retrospective cohort study and meta-analysis

First author and year Country Design N Preparation method Assay method FR cut-off value AR cut-off value Outcome
Fénichel 1991 [47] France Prospective cohort 41 Swim up GB24 >  0% vs. = 0%  
Takahashi 1993 [40] Japan Prospective cohort 45 Discontinuous gradient FITC-Con A <  50% %AR ≥ 10% at 2 h or ≤ 5% at 4 h
Parinaud 1993a [37] France Prospective cohort 53 Discontinuous gradient FITC-PNA >  0% vs. = 0%  
Parinaud 1993b [37] France Prospective cohort 53 Discontinuous gradient GB24 >  0% vs. = 0%  
Parinaud 1995 [41] Monaco Prospective cohort 117 Discontinuous gradient F1TC-GB24 Wholea  
Parinaud 1995 [42] Monaco Prospective cohort 131 Discontinuous gradient FITC-GB24 >  0% vs. = 0%  
Krausz 1996
[39]
Italy Prospective cohort 59 Swim up FITC-PNA ≥ 50% vs. <  50%  
Hershlag 1997
[44]
USA Prospective cohort 74 Swim up TRITC-PSA Whole  
Fujino 1997
[22]
Japan Prospective cohort 30 Discontinuous gradient + Swim up Two stain (new-methylene blue and cresyl violet) Blutstan kit Whole  
Rufas 1998
[64]
Israel Prospective cohort 62 Discontinuous gradient FITC-PSA >  0% vs. = 0%  
Kawamoto 1999
[29]
Japan Prospective cohort 34 Swim up MH61 >  0% vs. = 0%  
Bastiaan 2003
[63]
South Africa Prospective cohort 30 Swim up FITC-PSA >  50% vs. <  50%  
El-Ghobashy 2003
[33]
UK Prospective cohort 75 Swim up FITC-PSA ≥ 50% vs. <  50%  
Wiser 2014
[36]
Israel Prospective cohort 40 Not reported FITC-PSA ≤ 35% ≤10%
  1. AR acrosome reaction, FR fertilization rate, FITC-ConA fluorescein isothiocyanate-conjugated Concanavalin A lectin, FITC-PNA fluorescein isothiocyanate-conjugated peanut agglutinin, FITC-GB24 fluorescein isothiocyanate-GB24, TRITC-PSA tetramethylrhodamine-conjugated Pisurn sativum agglutimm, FITC-PSA fluorescein isothiocyanate-conjugated Pisurn sativum agglutimm
  2. aAll infertile patients undergoing in vitro fertilization (IVF) therapy
  3. spearman correlation coefficient; spontaneous AR% for higher and lower FR; binary diagnostic accuracy data as 2 × 2 tables