Fig. 4

Effect of ovary storage time at 4 °C on intracellular GSH (a) and ROS levels (b) of in vitro matured oocytes. Epifluorescence photomicrographs of MII oocytes that were stained with CellTracker Blue to determine the level of GSH (a’) and with 2′7′-dichlorodihydrofluorescein diacetate (H2DCFDA) to detect ROS (b’). Different superscripts (a, b, c, d) among storage times indicate P < 0.05. Scale bar =50 μm