Fig. 2

Three-dimensional and cross-sectional images of the ovary stained with PI. a-d Female mice were transcardially perfused with 4% PFA containing PI, and the isolated ovaries were subjected to CUBIC. Three-dimensional images were taken by light-sheet microscopy. a A 3D image of PI signals of the whole ovary. b A sectional image within the white square of (a). c A higher magnification image within the white square of (b). d A free-angle cross sectional image. gr, granulosa cells; atr, antrum; o, oocyte; th, thecal cells. Scale bars, 1 mm (a), 500 μm (b, d), and 200 μm (c)