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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: In vivo antral follicle wall biopsy: a new research technique to study ovarian function at the cellular and molecular levels

Fig. 3

Examples of histological sections of ex vivo follicle walls (Experiment 1) obtained from (a and b) small, (d and e) medium, and (g and h) large follicles using the FWB (a, d, g), and scalpel blade (b, e, h) techniques. For comparative purposes, the same follicle was sampled twice, i.e., with the FWB technique and the scalpel method. c, f, i immunofluorescence detection in walls of large follicles; c negative control; f ERRα; and (i) LHR. Granulosa, theca interna, and theca externa layers can be seen in the follicle wall samples. g, granulosa; ti, theca interna; and te, theca externa

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