Table 5 Gene ontology of predicted targets for heat-sensitive miRNAs found in round spermatids
From: The thermo-sensitive gene expression signatures of spermatogenesis
| Â | No. of gene | Name of genes |
|---|---|---|
Molecular functions | ||
 Binding | 7 | Pak7, Arhgef2, Cast, Tp63, Cast, Dazl, Wnt5b |
 Catalytic activity | 18 | Grip1, Ddx4, Mapk8, Rictor, Pak7, Arhgef2, Ddx6, Cast, Txnrd1, Mapk6, Cnot7, Dhx57, Arhgap1, Cybrd1, Map2k1, RragB, Cdk14 Gsk3a |
 Receptor activity | 1 | Calcr |
 Structural molecule activity | 1 | Slc25a43 |
 Translation regulator activity |  | Eif4e2,Eif4g2 |
 Transporter activity | 17 | Slc6a6, Slc38a11, Cacna1d, Slc38a2, Slc6a8, Slc13a5, Slc16a7, Slc30a7, Slc5a9, Slc35a2, Slc44a1, Slc17a5, Slc6a1, Slc23a2, Slc4a10, Slc20a2, Slc1a3 |
Biological functions | ||
 Biological adhesion | 1 | Arhgef2 |
 Biological regulation | 13 | Ddx4, Rictor, Pak7, Cacna1d, Ddx6, Tp63, Slc30a7, Cnot7, Wnt5b, Arhgap1, Map2k1, Slc4a10, RragB |
 Cellular component organisation or biogenesis | 3 | Rictor, Pak7, Ddx6 |
 Cellular process | 36 | Calcr, Slc6a6, Slc25a43, Grip1, Slc38a11, Ddx4, Slc12a6, Mapk8, Rictor, Pak7, Cacna1d, Slc38a2, Slc6a8, Arhgef2, Slc13a5, Ddx6, Slc16a7, Tp63, Slc8a3, Mapk6, Slc30a7, Slc5a9, Cnot7, Prkar2b, Dhx57, Wnt5b, Slc17a5, Arhgap1, Slc6a1, Map2k1 Slc4a10, RragB, Slc20a2, Cdk14, Slc1a3, Gsk3a |
 Developmental process |  | Calcr,Pak7, Notch4, Arhgef2, Tp63, Wnt5b Map2k1, Cdk14, Gsk3a,Eif4g2 |
 Immune system process | 2 | Mapk8, Mapk6 |
 Localization | 17 | Calcr, Slc6a6, Slc38a11, Pak7, Slc38a2, Slc6a8, Slc13a5, Slc16a7, Cast, Slc5a9, Slc35a2, Slc17a5, Slc6a1, Slc23a2, Slc4a10, Slc20a2, Slc1a3 |
 Locomotion | 1 | Pak7 |
 Metabolic process | 15 | Slc25a43, Ddx4, Ddx6, Cast, Tp63, Txnrd1, Slc35a2, Cnot7, Dhx57, Arhgap1, Slc23a2, RragB, Cdk14, Slc1a3, Gsk3a |
 Multicellular organismal process | 8 | Calcr, Grip1, Slc12a6, Wnt5b, Slc6a1, Cdk14, Slc1a3, Gsk3a |
 Reproduction | 2 | Calcr, Dazl |
 Response to stimulus | 10 | Calcr, Mapk8, Rictor, Pak7, Tp63, Mapk6, Slc30a7, Wnt5b, Map2k1, RragB |
Cellular components | ||
 cell junction | 1 | Grip1 |
 cell part | 23 | Slc6a6, Grip1, Ddx4, Rictor, Pak7, Cacna1d, Slc38a2,Slc6a8, Slc13a5, Ddx6,Slc16a7,Tp63, Slc30a7, Slc5a9, Cnot7, Dhx, Arhgap1,Slc6a1, Cybrd1, Map2k1,Slc4a10,RragB,Slc20a2 |
 extracellular region | 1 | Wnt5b |
 macromolecular complex | 6 | Ddx4, Rictor, Ddx6, Tp63, Cnot7, RragB |
 membrane transporter | 12 | Slc6a6, Slc38a2, Slc6a8, Slc13a5, Slc16a7, Slc5a9, Slc17a5, Slc6a1, Cybrd1, Slc4a10, RragB, Slc20a2 |
 Organelle | 9 | Ddx4, Slc38a2, Ddx6, Tp63, Slc30a7, Cnot7, Dhx57, Cybrd1, RragB |
Protein classes | ||
 calcium-binding protein | 1 | Slc25a43 |
 cell junction protein | 1 | Grip1 |
 defense/immunity protein | 1 | Calcr |
 enzyme modulator | 4 | Arhgef2, Cast,Arhgap1,RragB |
 membrane traffic protein | 1 | Cast |
 nucleic acid binding | 7 | Slc25a43, Ddx4, Ddx6, Eif4e2, Dazl, Dhx57, Eif4g2 |
 Oxidoreductase | 2 | Txnrd1, Cybrd1 |
 receptor | 1 | Calcr |
 signaling molecule | 1 | Wnt5b |
 transcription factor | 2 | Tp63, Cnot7 |
 transfer/carrier protein | 1 | Slc25a43 |
 transferase | 5 | Grip1, Mapk8, Mapk6, Cdk14, Gsk3a |
 transporter | 17 | Slc6a6, Slc25a43, Slc38a11, Cacna1d,Slc38a2 Slc6a8, Slc13a5,Slc16a7, Slc30a7, Slc5a9, Slc35a2, Slc44a1, Slc17a5, Slc6a1, Slc23a2, Slc4a10, Slc1a3 |
Pathways | ||
 5HT1 type receptor mediated signaling pathway | 1 | Prkar2b |
 5HT2 type receptor mediated signaling pathway | 1 | Cacna1d |
 Alzheimer disease-amyloid secretase pathway | 3 | Mapk8, Cacna1d, Mapk6 |
 Alzheimer disease-presenilin pathway | 2 | Notch4, Wnt5b |
 Angiogenesis | 6 | Mapk8, Notch4, Mapk6, Wnt5b, Arhgap1,Map2k1 |
 Angiotensin II-stimulated signaling through G proteins and beta-arrestin | 1 | Map2k1 |
 Apoptosis signaling pathway | 1 | Mapk8 |
 B cell activation | 2 | Mapk8, Map2k1 |
 Beta1 adrenergic receptor signaling pathway | 2 | Cacna1d, Prkar2b |
 Beta2 adrenergic receptor signaling pathway | 2 | Cacna1d, Prkar2b |
 CCKR signaling map | 2 | Mapk8, Map2k1 |
 Cadherin signaling pathway | 1 | Wnt5b |
 Cytoskeletal regulation by Rho GTPase | 2 | Pak7, Arhgap1 |
 Dopamine receptor mediated signaling pathway | 1 | Prkar2b |
 EGF receptor signaling pathway | 2 | Mapk8, Map2k1 |
 Endothelin signaling pathway | 2 | Prkar2b, Map2k1 |
 Enkephalin release | 1 | Prkar2b |
 FAS signaling pathway | 1 | Mapk8 |
 FGF signaling pathway | 2 | Mapk8,Map2k1 |
 GABA-B receptor II signaling | 1 | Prkar2b |
 Gonadotropin-releasing hormone receptor pathway | 4 | Mapk8,Cacna1d, Map3k7,Map2k1 |
 Hedgehog signaling pathway | 1 | Prkar2b |
 Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway | 2 | Prkar2b, Gsk3a |
 Histamine H2 receptor mediated signaling pathway | 1 | Prkar2b |
 Huntington disease | 1 | Tp63 |
 Inflammation mediated by chemokine and cytokine signaling pathway | 2 | Pak7, Map3k7 |
 Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade | 1 | Map2k1 |
 Ionotropic glutamate receptor pathway | 1 | Slc1a3 |
 Insulin/IGF pathway-protein kinase B signaling cascade | 1 | Gsk3a |
 Integrin signalling pathway | 3 | Mapk8, Mapk6, Map2k1 |
 Interferon-gamma signaling Pathway | 1 | Mapk8 |
 Interleukin signaling pathway | 2 | Map3k7, Mapk6 |
 Muscarinic acetylcholine receptor 2 and 4 signaling pathway | 2 | Slc6a8, Prkar2b |
 Metabotropic glutamate receptor group III pathway | 2 | Prkar2b, Slc1a3 |
 Metabotropic glutamate receptor group II pathway | 1 | Prkar2b |
 Nicotinic acetylcholine receptor signaling pathway | 2 | Cacna1d, Slc6a8 |
 Notch signaling pathway | 2 | Notch4, Gsk3a |
 Oxidative stress response | 1 | Mapk8 |
 Oxytocin receptor mediated signaling pathway | 1 | Cacna1d |
 P53 pathway feedback loops 1 | 1 | Tp63 |
 PDGF signaling pathway | 4 | Mapk8, Mapk6,Arhgap1,Map2k1 |
 Parkinson disease | 1 | Mapk8 |
 Ras Pathway | 3 | Mapk8,Map2k1,Gsk3a |
 T cell activation | 2 | Mapk8,Map2k1 |
 TGF-beta signaling pathway | 2 | Mapk8, Map3k7 |
 Toll receptor signaling pathway | 3 | Mapk8, Map3k7, Map2k1 |
 Transcription regulation by bZIP transcription factor | 1 | Prkar2b |
 VEGF signaling pathway |  | Mapk6, Arhgap1, Map2k1 |
 Wnt signaling pathway |  | Map3k7,Wnt5b |
 p38 MAPK pathway |  | Map3k7 |
 p53 pathway by glucose deprivation |  | Tp63 |
 p53 pathway feedback loops 2 |  | Tp63 |
 p53 pathway |  | Tp63 |