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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: MicroRNA 21a-5p overexpression impacts mediators of extracellular matrix formation in uterine leiomyoma

Fig. 2

Gene and protein expression of TGF-β family. All results are presented as fold change with NTC as the reference, mean ± SEM, and P-value < 0.05 is indicated by an asterisk. a Expression of TGF-β Family Genes. Gene target RT-PCR relative expression of TGF-β family genes in fibroid and myometrial cells upregulated with miR-21. Results represent the mean of three independent experiments from each of three independent infections. b TGF-β3 protein expression. Western Blot protein expression of TGF-β3 in fibroid and myometrial cells upregulated with miR-21. GAPDH is shown as loading control. Graph shows TGF-β3 protein expression (corrected for GAPDH levels) in miR-21 upregulated fibroid and myometrium is normalized to each tissue’s respective NTC (corrected for GAPDH levels). Results represent the mean of three independent experiments

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