Fig. 2

Gene and protein expression of TGF-β family. All results are presented as fold change with NTC as the reference, mean ± SEM, and P-value < 0.05 is indicated by an asterisk. a Expression of TGF-β Family Genes. Gene target RT-PCR relative expression of TGF-β family genes in fibroid and myometrial cells upregulated with miR-21. Results represent the mean of three independent experiments from each of three independent infections. b TGF-β3 protein expression. Western Blot protein expression of TGF-β3 in fibroid and myometrial cells upregulated with miR-21. GAPDH is shown as loading control. Graph shows TGF-β3 protein expression (corrected for GAPDH levels) in miR-21 upregulated fibroid and myometrium is normalized to each tissue’s respective NTC (corrected for GAPDH levels). Results represent the mean of three independent experiments