Table 1 Primer pairs specific for each gene used in the study
From: Deficiency of Gpr1 improves steroid hormone abnormality in hyperandrogenized mice
Name | Fw Primer Sequence (5′-3′) | Rv Primer Sequence (5′-3′) | Target Sequence |
|---|---|---|---|
GnRH | gtggatcaaatggcagaaccc | gggccagtgcatctacatct | NM_008145.3 |
AR | tccaagacctatcgaggagcg | gtgggcttgaggagaaccat | NM_013476.4 |
ERA | cccgccttctacaggtctaat | ctttctcgttactgctggacag | NM_007956.5 |
LH | tggccgcagagaatgagttc | ctcggaccatgctaggacagtag | NM_008497.2 |
FSH | ggagagcaatctgctgccata | gcagaaacggcactcttcct | NM_008045.3 |
GH | ctacaaagagttcgagcgtgcctac | caattccatgtcggttctctgc | NM_008117.3 |
PRL | agggagttgagaagataattagccag | aagaggagaccaattgcaccca | NM_011164.2 |
UCP1 | actgccacacctccagtcatt | ctttgcctcactcaggattgg | NM_009463.3 |
StAR | ccgggtggatgggtcaa | cacctctccctgctggatgta | NM_011485.4 |
P450scc | ccatcagatgcagagtttccaa | tgagaagagtatcgacgcatcct | NM_019779.3 |
3B-HSD | ggaggcctgtgttcaagcaa | ggccctgcaacatcaactg | NM_008293.4 |
17B-HSD | ttgtttgggccgctagaag | cacccacagcgttcaattca | NM_010475.1 |
Aromatase | gcaatcctgaaggagatcca | gccgtcaattacgtcatcct | NM_007810.3 |
Hsd17b7 | cctgtgctcagtccgttttt | ccaaggccctgaattcaata | NM_010476.3 |