Fig. 5
From: Deficiency of Gpr1 improves steroid hormone abnormality in hyperandrogenized mice
Granulosa cells were isolated after PMSG treatment for 48 h and hCG treatment for 7–12 h and further treated under different conditions for 24 h. A Cell Proliferation under different conditions. B Estradiol levels in culture media from cells under different conditions. C, D Relative mRNA levels for Aromatase and HSD17B7 in granulosa cells under different conditions. Data were analyzed by an unpaired Student’s t test. (a) p, DHEA-treated vs. control IgG group; (b) p, DHEA-treated vs. control GPR1 antibody group; (c) p, control GPR1 antibody group vs. control IgG group; (d) p, DHEA-treated GPR1 antibody group vs. DHEA-treated IgG group. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)