Fig. 4

Ovarian features and expression of mRNAs for steroid synthesis enzymes in CTL and DHEA-treated wild type and Gpr1-null mice. A Hematoxylin and eosin staining of CTL and DHEA-treated ovaries was examined. The high magnification images on the right show the difference of ovary morphology among various treatment groups. AF, antral follicle. CL, corpus luteum. CF, Cystic follicle. TC, theca cell. GC, granulosa cell. F, follicle. Data represent means ± SEM. Bar = 200 μm. B Expression of mRNA for steroid synthesis enzymes in CTL and DHEA-treated wild type and Gpr1-null mice. Data were analyzed by an unpaired Student’s t test. (a) p, DHEA-treated vs. control wild type mice; (b) p, DHEA-treated vs. control Gpr1-null mice; (c) p, control Gpr1-null mice vs. control wild type mice; (d) p, DHEA-treated Gpr1 null mice vs. DHEA-treated wild type mice