Fig. 2

Antibody levels of ZP3 and GnRH in serum. Sera were collected from the mice on day 0, 14, 35, 56 and 168 after the 1st immunization. a. Ab levels of ZP3. All the serum samples were diluted (1:1000) with PBST containing 5% skim milk. HRP-conjugated goat anti-mouse IgG Ab (1:1000) was used as the second Ab. b. Ab levels of GnRH. Serum samples were collected from the mice on day 14, 56 and 168 at 1:100 dilution. Data are shown as mean ± SEM. ***P<0.001