Parental genetic material and oxygen concentration affect hatch dynamics of mouse embryo in vitro

Table 2 Cell allocation and apoptosis in fertilized and parthenogenetic embryo cultured in vitro for 120 h

Groups	No. embryos	120 h Blastocysts⁺			No. embryos	120 h Blastocysts
Groups	No. embryos	No. total cells	No. Oct4 positive cells	Oct4 positive cells/Total cells (%)	No. embryos	No. apoptosis cells
HFE	37	116.0 ± 3.2^a	25.2 ± 1.2	21.7 ± 0.8^e	14	1.9 ± 0.6
LFE	39	129.4 ± 4.4^b	24.5 ± 1.2	18.8 ± 0.6^f	13	1.2 ± 0.6
HPA	49	105.7 ± 2.6^c	23.2 ± 1.0	21.9 ± 0.7^e	11	1.4 ± 0.4
LPA	60	112.6 ± 2.7^acd	27.6 ± 0.9	24.7 ± 0.6^g	12	1.3 ± 0.4

HFE and LFE fertilized embryos cultured in 20 and 5% oxygen, HPA and LPA parthenogentic embryos cultured in 20 and 5% oxygen
⁺Mean ± SEM
^a vs. ^b, p < 0.01; ^a vs. ^c, p < 0.05; ^b vs. ^c, p < 0.0001; ^b vs. ^d, p < 0.001; ^c vs. ^d, p < 0.05; ^e vs. ^f, ^g P < 0.01; ^f vs. ^g, P < 0.01

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