Fig. 6
Transcript analysis of neurotransmitter receptor in PCOS rats. mRNA expression profile of a serotonin 5HT1A receptor (5HT1A); b alpha1-adrenergic receptor (α 1 AR); c dopamine D2 receptor (D 2 R); d GABA B1 receptor (GABA B1 ); e muscarinic acetylcholine 2 receptor (M 2 AchR) and f NMDA glutamate receptor (NMDA) in control and PCOS brain tissues. Bar graph represents values of mean fold change in gene expression of PCOS animals as compared to control rats (represented by black dashed line). β-Actin was used as internal control for mRNA studies and fold change in expression was calculated by 2-ΔΔCT method. Error bars represent SEM; n = 6 per group; *P < 0.05; **P < 0.01; ***P < 0.001 as compared to control group