Fig. 5

Decrease of ODPFs in oocytes was mediated by CCs. a Schematic diagram of the culturing system. b–d The relative mRNA levels of Gdf9 (b), Bmp15 (c) and Fgf8 (d) in oocytes respectively from DOs, COCs and “OOX + DO” group were determined by real-time q-PCR cultured in medium with 100 ng/ml NGF for 18 h. DO, denuded oocyte; oocyte/COC, oocytes from COC group; oocyte/OOX + DO, oocytes from the co-cultured OOX-cumulus cells and denuded oocytes group. ≥35 oocytes were used for RNA extraction in each group. Experiments were repeated at least three times independently. Data are presented as mean ± SEM. Student’s two-tailed t-test was used for statistical analysis *, p < 0.05; **, p < 0.01, ***, p < 0.001