Skip to main content

Table 2 Primers for real-time quantitative polymerase chain reaction (PCR)

From: ART manipulation after controlled ovarian stimulation may not increase the risk of abnormal expression and DNA methylation at some CpG sites of H19,IGF2 and SNRPN in foetuses: a pilot study

Gene

Primer sequence(5’to3’)

Product size(bp)

H19

ID:283120

Forward

TCAAAGCCTCCACGACTCTGT

88

Reverse

GCCGTCTCCACAACTCCAA

IGF2

ID:3481

Forward

TGAGATCCAAAACGCTTCGA

87

Reverse

CGGCGAGGCAGAATATAACAC

SNRPN

ID:6638

Forward

CGTCCACCAAGACCTTAGCATAC

100

Reverse

AACAAAAAGCTCACAAACACTCTACAC

Gapdh

ID:2597

Forward

TGACTTCAACAGCGACACCCA

100

Reverse

CACCCTGTTGCTGTAGCCAAA