Fig. 4

Effects of L-lactate transport inhibitors on L-lactate induced changes of hormone and gene expression profile. GC were cultured as described before and additionally pre-treated for two days with the inhibitor for L-lactate transporters, UK5099 (50 μM). (a) Inhibition of L-lactate transporters (black bars) resulted in the reversal of the L-lactate effect (dark grey bars) on E2 production. (b) The gene expression levels of selected transcripts nearly regained control levels (dashed line) after inhibition of L-lactate transporters, whereas the inhibitor alone showed nearly no effects (light grey bars). Transcript abundance was normalized to the reference gene RPLP0. Means and SEM of the hormone concentration and gene expression are shown of n = 4 replicates. The student’s t-test or Mann-Whitney test was used for analyzing the effects compared to the vehicle control (*) and between different treatments the ANOVA testing was used (indicated by letters), n = 4, P < 0.05.