Fig. 4
From: Expression of cystatin C in the female reproductive tract and its effect on human sperm capacitation
Effects of CST3 on human sperm motility. Sperm isolated by density gradient was incubated in EBSS medium, or the medium was supplemented with BSA, CST3, or BSA and CST3 (see text for details) at 37 °C under 5% (v/v) CO2 for 3 h. Motility parameters, including motile sperm percentage (a), progressively motile sperm percentage (b), VCL (c), and STR (d), at each specified incubation time were determined. Points are expressed as mean ± SD of eight independent determinations. *p < 0.05 and **p < 0.01 vs. the control (CTR) group; #p < 0.05 vs. the BSA group at corresponding time points