Fig. 3

Effect of AKT inhibitors III and IV treatments on AKT-Thr308 phosphorylation levels in early bovine embryos. Presumptive zygotes were cultured in presence of 0, 25, 50 or 75 μM AKT inhibitor III or 0, 1.5, 2.5 or 3.5 μM AKT inhibitor IV for 10 h, then subjected to Western blot for phosphorylated (p)AKt-Th308, total AKT (tAKT) and actin analysis (n = 3 replicates, n = 20 embryos/treatment). Data were normalized relative to abundance of actin and phosphorylation levels were expressed as pAKT/tAKT (a, b). Representative Western blot images are shown. Data are expressed as mean ± SEM. Values with different superscripts among treatments indicate significant differences (P < 0.05)