Fig. 3

Histone modifications of inactive chromatin in αT1–1, αT3–1, and LβT2 cells. ChIP analysis was performed using antibodies specific to H3K27Me3, and H3K9Me2. Immunoprecipitated chromatin from αT1–1, αT3–1, and LβT2 cells was subjected to qRT-PCR. Data are presented as fold enrichment relative to IgG, with values normalized to Actb and expressed as mean ± SEM. Statistical analysis by one-way ANOVA followed by a Tukey post-hoc, * p < 0.05, ** p < 0.01, *** p < 0.001, as indicated by bar. n.s.: non-significant