Fig. 2

Active histone modifications and recruitment of phospho-Pol II to gonadotrope-specific genes in gonadotrope cells. ChIP analysis using antibodies specific to (a) H3Ac, (b) phospho-Pol II, and (c) H3K4Me3 in αT1–1, αT3–1, and LβT2 cells. Immunoprecipitated chromatin from αT1–1, αT3–1, and LβT2 cells was subjected to qRT-PCR. Data are presented as fold enrichment relative to IgG, with values normalized to Gnrh1 and expressed as mean ± SEM (n = 4). Statistical analysis by one-way ANOVA followed by a Tukey post-hoc, * p < 0.05, ** p < 0.01, *** p < 0.001, as indicated by bar. n.s.: non-significant