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Table 4 Morphometric parameters after thawing for survived slow frozen and vitrified embryos in dataset 1. Differences between survived slow frozen and vitrified embryos, and between day of evaluation, were calculated for each parameter using linear regression (95% CI and p values)

From: Effect of day 3 embryo morphometrics and morphokinetics on survival and implantation after slow freezing-thawing and after vitrification-warming: a retrospective cohort study

  Day of
measurement
Slow frozen
Mean ± SD
Vitrified
Mean ± SD
Coefficient (95%CI)
Freezing method
P value
Freezing method
Coefficient (95% CI)
Day
P value
Day
N embryos   52 a 80 b, c     
TCV (μm3) At thaw/warming 550,798 ± 162,603 702,510 ± 12,793 −143,081
(193,476 to −92,686)
<0.0001* −65,045
(−102,621 to −27,468)
0.0008
After O/N culture 505,520 ± 144,231 607,813 ± 89,982
Blastomeres (n) At thaw/warming 7.1 ± 2.6 8.3 ± 2.3 −0.98
(−2.05 to 0.09)
0.0729 1.58
(0.89 to 2.26)
<0.0001
After O/N culture 8.5 ± 3.4 9.6 ± 3.0
Symmetry (%) At thaw/warming 76.4 ± 8.7 72.4 ± 9.6 3.97
(0.77 to 7.18)
0.0155* −6.46
(−8.82 to −4.09)
<0.0001
After O/N culture 70.5 ± 8.9 66.4 ± 6.7
  1. *Statistically significant (at 5% alpha level) using the Bonferroni-Holm correction for multiple testing to test for any difference between slow frozen and vitrified embryos
  2. Random intercept correction was used to account for clustering
  3. a14/52 embryos were compacted after overnight culture
  4. b3/83 embryos were compacted after thawing
  5. c50/83 embryos were compacted after overnight culture