Effect of day 3 embryo morphometrics and morphokinetics on survival and implantation after slow freezing-thawing and after vitrification-warming: a retrospective cohort study

Table 4 Morphometric parameters after thawing for survived slow frozen and vitrified embryos in dataset 1. Differences between survived slow frozen and vitrified embryos, and between day of evaluation, were calculated for each parameter using linear regression (95% CI and p values)

	Day of measurement	Slow frozen Mean ± SD	Vitrified Mean ± SD	Coefficient (95%CI) Freezing method	P value Freezing method	Coefficient (95% CI) Day	P value Day
N embryos		52 ^a	80 ^{b, c}
TCV (μm³)	At thaw/warming	550,798 ± 162,603	702,510 ± 12,793	−143,081 (193,476 to −92,686)	<0.0001*	−65,045 (−102,621 to −27,468)	0.0008
TCV (μm³)	After O/N culture	505,520 ± 144,231	607,813 ± 89,982	−143,081 (193,476 to −92,686)	<0.0001*	−65,045 (−102,621 to −27,468)	0.0008
Blastomeres (n)	At thaw/warming	7.1 ± 2.6	8.3 ± 2.3	−0.98 (−2.05 to 0.09)	0.0729	1.58 (0.89 to 2.26)	<0.0001
Blastomeres (n)	After O/N culture	8.5 ± 3.4	9.6 ± 3.0	−0.98 (−2.05 to 0.09)	0.0729	1.58 (0.89 to 2.26)	<0.0001
Symmetry (%)	At thaw/warming	76.4 ± 8.7	72.4 ± 9.6	3.97 (0.77 to 7.18)	0.0155*	−6.46 (−8.82 to −4.09)	<0.0001
Symmetry (%)	After O/N culture	70.5 ± 8.9	66.4 ± 6.7	3.97 (0.77 to 7.18)	0.0155*	−6.46 (−8.82 to −4.09)	<0.0001

*Statistically significant (at 5% alpha level) using the Bonferroni-Holm correction for multiple testing to test for any difference between slow frozen and vitrified embryos
Random intercept correction was used to account for clustering
^a14/52 embryos were compacted after overnight culture
^b3/83 embryos were compacted after thawing
^c50/83 embryos were compacted after overnight culture

ISSN: 1477-7827