Fig. 4

Effect of GSK461364 on cell cycle progression in porcine oocytes undergoing meiotic progression. (a) Representative images of porcine oocytes after culture with or without GSK461364 from 28 to 44 h in vitro. Most of the control oocytes reached MII stage while a larger proportion of Plk1-inhibited oocytes were arrested at ATI stage. Blue, chromosome. Scale bar, 80 μm. (b) The proportion of oocytes at different stages. Most oocytes were arrested at ATI stage after Plk1 inhibition. Compared to the control group, the percentage of Plk1-inhibited oocytes that reached MII stage was sharply decreased, whereas the proportion of oocytes that were arrested at ATI stage was significantly increased. * P < 0.05; *** P < 0.001