Table 1 Characteristics of real-time RT PCR
Gene symbol | GenBank accession No. | Forward primer/Reverse primer/Probe | Primer/probe size (bp) | Amplicon size (bp) |
|---|---|---|---|---|
ACTB | AY141970.1 | 5’CGTCCACCGCAAATGCTT’3 5’TCTCGTTTTCTGCGCAAGTTAG’3 5’TAGGCGGACTGTTAGC’3 | 18 22 16 | 85 |
LPAR1 | NM_174047.2 | 5’GTTCAACACAGGGCCCAATACT’3 5’AACCGTCAGGCTGGTGTCA’3 5’TGACTGTCAGCACGTGGCTCCTGC’3 | 22 19 24 | 85 |
LPAR2 | NM_001192235.1 | 5’GTCTCTGAGCCTGCGGGTTA’3 5’TGACTTCAGAGCCCAATTCTTG’3 5’CATCTATGCAACTGGGAG’3 | 20 22 18 | 80 |
LPAR3 | NM_001192741.2 | 5’TCAAGTAAAGCACGGGCAGAA’3 5’CGGGTGAGAACGCATTGTG’3 5’AGGACACGTGCATTTACAGA’3 | 21 19 20 | 90 |
LPAR4 | NM_001098105.1 | 5’ATCACCAATCTGGCCCTCTCT’3 5’CAGTGGCGGTTGAAATTGTAAAA’3 5’CTCTTTGTCTGCACTCTA’3 | 21 23 18 | 80 |
AX | NM_001080293.1 | 5’CCATTATTCCCACAAGTTCACAGTA’3 5’TGTCAAGCATGTCACCAAAGGT’3 5’TGCCACAAAAGTGACAAA’3 | 19 20 18 | 80 |
PLA2 | NM_001075864.1 | 5’CCATTATTCCCACAAGTTCACAGTA’3 5’TGTCAAGCATGTCACCAAAGGT’3 5’TGCCACAAAAGTGACAAA’3 | 25 22 18 | 80 |
TNFα | AC_000180.1 | 5’CCCTTCCACCCCCTTGTT’3 5’TGATGGCAGACAGGATGTTGA’3 5’CTCACCCACACCATCAGCCGCA’3 | 18 21 22 | 85 |
TNFα R1 | U90937.1 | 5’AGAGACAGGACACCATCTGC’3 5’GCAAAGCCCGAAGACAATCA’3 5’ATGTCCAACCCGACCTTCAA’3 | 20 20 20 | 188 |
TNFα R2 | NM_001040490.2 | 5’ATAGTGGGGAAAGGACAGGC’3 5’AGGAGTGGATTTGGAGGTGG’3 5’GGTACCCCAGTTAGCTCAGG’3 | 20 20 20 | 175 |
FAS | NM_174662.2 | 5’GCTAAGTCGCTTCAGTCGTG’3 5’GGATTGCTGAGTCGGACATG’3 5’CTGGAGTGGGTTGCCATTTC’3 | 20 20 20 | 179 |
FasL | NM_001098859.1 | 5’AGTACAAGAAGGGCAGCCTT’3 5’TCATCTTCCCCTCCATCAGC’3 5’GTCAGTCCTGCAACAACCAG’3 | 20 20 20 | 165 |
BCL2 | U92434.1 | 5’TCTTTGAGTTCGGAGGGGTC’3 5’GGCCATACAGCTCCACAAAG’3 5’ATGACCGAGTACCTGAACCG’3 | 20 20 20 | 162 |
BAX | U92569.1 | 5’AACATGGAGCTGCAGAGGAT’3 5’CCAATGTCCAGCCCATGATG’3 5’AGTGGCGGCTGAAATGTTTT’3 | 20 20 20 | 208 |
CASP3 | NM_001077840.1 | 5’TCTAAGCCATGGTGAAGAAGGAAT’3 5’GTCCCCTCTGAAGAAACTTGCTAA’3 5’TTTTGGAACCAACGGACCCGTCAA’3 | 24 24 24 | 84 |
CASP8 | DQ319070.1 | 5’CTACTGAGAGAAGAGGCCCG’3 5’TTGATGGGTTCCTGCTTCCT’3 5’CTCAAGTTCCTAAGCCGGGA’3 | 20 20 20 | 210 |
B-glycan | NC_005113.4 | 5’TTCCAGGACGAACCCAACA’3 5’CCTCAACATAAACGTGTCCATTCT’3 5’CGTCACCTTCAACATGGAGCTGTACAACAC’3 | 19 24 30 | 86 |
DRAK2 | AC_000159.1 | 5’TAACAGAGCAAATCGGACACAAG’3 5’ATTCCGCTTTGACGACTCGCTGCC’3 5’AGCAGCACGGTGTCCAAGA’3 | 22 24 19 | 79 |
MCL1 | NM_001099206.1 | 5’CCAGGCTCGGACGGCTCGC’3 5’CTTTACCTTTGTTGGTCGGAGAA’3 5’TCTCCAGGGACTGCCGATAT’3 | 19 23 20 | 82 |
ERβ | NM_001001443.1 | 5’GCTGTCAGGCCTGTCGACTT’3 5’ATGCGGTACCCACACCTTTCT’3 5’CTATGAGGTTGGAATGGT’3 | 20 21 18 | 85 |
DICE1 | AY386968.1 | 5’ACCGCCTTCACTGCCTTCT’3 5’AGATGAAAGATTTGCTGTCGATAGATT’3 5’CAGAGCTCGGACGGCTCGC’3 | 19 27 19 | 85 |
SOX2 | AC_000158.1 | 5’CACCTGCAAGTTTCCTGCTT’3 5’TGTAAAATGTGTAGCCGCCG’3 5’CAGCATATTGACTCCACCGC’3 | 20 20 20 | 202 |