Fig. 2

mESCs differentiate to express endometrial precursor cell markers CD9 and CD13 by three weeks in standard culture. By one, two, and three weeks in culture, mESCs display both human endometrial epithelial CD9 (b, f, j) and stromal CD13 cell markers (c, g, k). Co-expression is illustrated in (d, h, and l). As shown in (m), colonies of mESCs showed progressive differentiation of CD9 immunoreactive cells over a three-week period (58%, 60%, and 31% in weeks one, two, and three, respectively), while populations of differentiated CD13 cells increased over this same duration (57%, 34% and 62% in weeks one, two, and three, respectively). Bottom right scale bar in panel (l) indicates 20 μm for all panels