Improving preimplantation genetic diagnosis (PGD) reliability by selection of sperm donor with the most informative haplotype

Table 3 Screening various sperm donors’ genotypes for a representative PGD patient with Huntington disease

Markers	chr4: 1,953,007–1,953,486^b	chr4: 2,351,587–2,352,006	chr4: 3,038,646– 3,039,039	chr4: 3,052,695–3,053,077	chr4: 3,076,297–3,076,716	chr4: 3,085,088–3,085,500	chr4: 3,325,450–3,325,833	chr4: 3,230,879–3,231,097	chr4: 3,377,889–3,378,176
Patient #17	231/235	235/239	390/396	381/389	276/337	271/281	373/377	189/204	419/428
Donor 1	235/240	235/235	390/396	381/389	276/276	269/281	375/375	189/204	419/421
Donor 2	227/241	233/239	394/396	387/391	283/286	277/284	375/378	189/204	402/419
Donor 3	227/241	229/235	389/391	381/385	280/286	271/277	373/375	206/208	402/421
Donor 4	220n231	233/233	394/400	385/387	286/286	277/285	369/373	204/206	423/421
Donor 5^a	222/222	233/233	390/400	383/385	280/286	283/285	369/377	173/189	419/421

The numbers represent the length of amplified markers in bp, as read by a fragment analyzer
The bold numbers represent the markers’ lengths linked to the mutant allele
The donor’s alleles that differ from the subject’s normal alleles by ≤2 bp (considered as “overlapping”) are written in italics. Alleles’ differences >2 bp. in normal alleles (not overlapping) are underlined
^aSelected as the most suitable donor
^bBased on UCSC Genome Browser on Human Feb. 2009 (GRCh37/hg19) Assembly

ISSN: 1477-7827