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Table 3 Screening various sperm donors’ genotypes for a representative PGD patient with Huntington disease

From: Improving preimplantation genetic diagnosis (PGD) reliability by selection of sperm donor with the most informative haplotype

Markers chr4: 1,953,007–1,953,486b chr4: 2,351,587–2,352,006 chr4: 3,038,646– 3,039,039 chr4: 3,052,695–3,053,077 chr4: 3,076,297–3,076,716 chr4: 3,085,088–3,085,500 chr4: 3,325,450–3,325,833 chr4: 3,230,879–3,231,097 chr4: 3,377,889–3,378,176
Patient #17 231/235 235/239 390/396 381/389 276/337 271/281 373/377 189/204 419/428
 Donor 1 235/240 235/235 390/396 381/389 276/276 269/281 375/375 189/204 419/421
 Donor 2 227/241 233/239 394/396 387/391 283/286 277/284 375/378 189/204 402/419
 Donor 3 227/241 229/235 389/391 381/385 280/286 271/277 373/375 206/208 402/421
 Donor 4 220n231 233/233 394/400 385/387 286/286 277/285 369/373 204/206 423/421
 Donor 5a 222/222 233/233 390/400 383/385 280/286 283/285 369/377 173/189 419/421
  1. The numbers represent the length of amplified markers in bp, as read by a fragment analyzer
  2. The bold numbers represent the markers’ lengths linked to the mutant allele
  3. The donor’s alleles that differ from the subject’s normal alleles by ≤2 bp (considered as “overlapping”) are written in italics. Alleles’ differences >2 bp. in normal alleles (not overlapping) are underlined
  4. aSelected as the most suitable donor
  5. bBased on UCSC Genome Browser on Human Feb. 2009 (GRCh37/hg19) Assembly