Fig. 5
Steroid intermediates repress CRE reporter activity in Y1-BS1 cells. Y1-BS1 cells were transfected with Cre-Luc (firefly luciferase) (a) or pLuc-MCS vector (control) (b) and Renilla luciferase (Rluc, pRL-TK) for 24 h. Cells were then cultured in F-12K medium supplemented with 10% LPDS for 10 h before incubating with HDL3 (30 μg/ml), 22(R)-diol (10 μM) or pregnenolone (10 μM) for 1, 3 and 5 h followed by measurement of firefly and Renilla luciferase activities. Results are summary of three independent experiments with triplicates in each experiment. * p < 0.05