Fig 3

Blockade of angiogenin neutralized MSC-stimulated follicular survival after co-transplantation of MSCs. a Representative images of HE-stained section showing number of primodial follicles in ovarian tissue at post-transplant day-7 of each group in the shANG blockage experiment. b Quantitative analysis of follicular numbers in different groups in shANG blockage experiment. c Representative images of HE-stained section showing number of primodial follicles in ovarian tissue at post-transplant day-7 of each group in antibody blockage experiment. d Quantitative analysis of primodial follicular numbers in different groups in antibody blockage experiment. Data shown are means ± SEM of triplicates in a representative experiment. MSC, mesenchymal stem cells; ANG, angiogenin; CTRL, control; Ab, antibody; shANG, ANG specific short hairpin RNA; shCTRL, control short hairpin RNA, * P < 0.05. Scale bar = 50 μm