Fig. 5

G-CSF treatment promotes proliferation of PLZF+ spermatogonia after busulfan treatment. Result are from mice in Experiment 4. a-d Testis sections were co-stained for PLZF (green) and Ki67 (red) to determine the percentage of PLZF+ spermatogonia that were proliferating (Ki67+). Red arrowheads point to PLZF+/Ki67- cells and yellow arrowheads mark PLZF+/Ki67+ cells. The asterisk notation above bars denotes a statistically significant increase (p ≤0.0352) in the Ki67 labeling index of PLZF+ spermatogonia as determined by Student’s t-test. Scale bars = 50 μm