Fig. 2

Hormone concentrations in GC cultured at different plating densities. Estradiol (E2) concentrations significantly decreased when GC were cultured at high cell density (black bars) compared to cells at normal density (grey bars). On the other hand the progesterone (P4) concentration tended to increase at high cell density. Hormone concentrations (ng/ml) are normalized to total RNA amounts (ng) of cell preparations to correct for cell numbers; mean values and SEMs are shown (n = 3, P < 0.05, t-test)