Fig. 5

Proteomic analysis of bovine endometrial ICAR-derived exosomes. Mass spectrometric analyses of ICAR cell-derived exosome proteins. a Representative Venn diagram of common and unique proteins identified by 5600 Triple TOF MS (ABSciex) from exosomes released by ICAR cells at 48 h at both 8 % O₂ and 1 % O₂. b The gene ontology classification of ICAR cell-derived exosome proteins, on the basis of their involvement in biological process, identified clusters that are unique to and present only in exosomes of ICAR cultured at 1 % O₂ but not those at 8 % O₂. These biological processes were: growth (0.7 %), locomotion (0.7 %) and reproduction (1.4 %). c Molecular function (using PANTHER and Gene Ontology algoritnms) of exosome proteins were mostly related to binding and catalytic activity in both ICAR cultured at 1 % O₂ and at 8 % O₂