Table 1 Oligonucleotide sequences used for RT-PCR analysis
From: Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro
Marker | Forward 5'-3' | Reverse 5'-3' | T annealing | bp |
|---|---|---|---|---|
Interleukin-1β (IL-1β) | TGCAGCTGGAGGAAGTAGAC | GTCGGGCATGGATCAGACAA | 60 | 338 |
Interleukin 8 (IL-8) | ACATACCCTGCCACAAGGC | TGGGGACTGCTCTTCCCTCT | 57 | 146 |
Inducible nitric oxide synthase (iNOS) | GGACCTCAACAAAGCCCTGA | CCTTGACCCAATAGCTGCCA | 60 | 293 |
Prostaglandin-endoperoxide synthase 2 (COX2) | CATGGGTGTGAAAGGGAGGAA | AAAGACGTCAGGCAGAAGGG | 60 | 700 |
Tumor protein p53 (TP53) | CCTAGGAGCACTAAGCGAGC | GCCCCTCTCTCTTGAGCATT | 55 | 268 |
Oestrogen receptor alpha (ERα) | AGGGAAGCTCCTATTTGCTCC | CGGTGGATGTGGTCCTTCTCT | 55 | 234 |
Oestrogen receptor beta (ERβ) | GCTTCGTGGAGCTCAGCCTG | AGGATCATGGCCTTGACACAG | 55 | 262 |
Progesterone receptor (PR) | GAGAGCTCATCAAGGCAATTGG | CACCATCCCTGCCAATATCTTG | 55 | 227 |
v-myc avian myelocytomatosis viral oncogene homolog (c-Myc) | GCGCCGCATTCGCGAAACTT | TGAGGGGCATCGCTGCAAGC | 58 °C | 214 |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | ATGAGATCAAGAAGGTGGTG | CCAAATTCATTGTCGTACCAG | 60 | 190 |